Recent development in the X-ray studies
There are many different developments made like X-ray free-electron lasers (XFELs) that give different possibilities for X-ray crystallographic, spectroscopic studies of different radiation and sensitive biological samples that close to physiological conditions. To use these new X-ray methods, tailored experimental methods, data-processing protocols have to be imitated. The highly radiated-sensitive photosystem II (PSII) protein complex is a main target used for XFEL experiments used to study the mechanism of light-induced in water oxidation taking place at a Mn cluster in the complex. Then there was development of a set of tools for the study of PSII at XFELs, using a new liquid jet based on electrocuting, energy dispersive von Hamos the X-ray emission spectrometer for the hard X-ray range and also high-throughput soft X-ray spectrometer up on a reflection zone plate. But our next focus is on PSII, that the methods we use here are applicable to a wide range of metalloenzymes.
- Quantitative analysis
- Crystal structure refinement by the Rietveld method
- Stress, Strain and Crystallite size determination
- Small angle scattering
- Quantum Crystallography
- Biological Small-Angle Neutron Scattering (Bio-SANS)
- Small-angle X-ray scattering (SAXS)
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